Journal: Advanced Science

Article Title: Molecular Design of a Naturally Derived Hemostatic Sealant with Prolonged Antimicrobial Activity for Repairing Elastic Organ Injuries

doi: 10.1002/advs.202506466

Figure Lengend Snippet: In vitro antibacterial properties and biocompatibility of GDP sealant. In vitro antimicrobial tests: A) Bacterial concentration measured by optical density (OD) at 625 nm of P. aeruginosa during 5 days of culture with either GDP hydrogels prepared with various concentrations of DMA and pDDA, broad‐spectrum antibiotic ciprofloxacin, or commercial wound dressing AquaDerm. B) Survival rate of P. aeruginosa after 5 days of treatment. C) Representative SEM images of GelMAG and GDP surfaces after 5 days of bacterial inoculation. D) Zone of inhibition (ZOI) formed by GelMAG or GDP against P. aeruginosa . E) OD measurements of MRSA during 5 days of culture with either GDP hydrogels prepared with various concentrations of DMA and pDDA, broad‐spectrum antibiotic ciprofloxacin, or commercial wound dressing AquaDerm. F) Survival rate of MRSA after 5 days of treatment. G) Representative SEM images of GelMAG and GDP surfaces after 5 days of bacterial inoculation. H) ZOI formed by GelMAG or GDP against MRSA. Negative control represented bacteria without hydrogel treatments. In vitro biocompatibility assessment: I) Representative live/dead stained images from NIH3T3 cells exposed to GelMAG or GDP through Transwell ® inserts for 5 days. Control cells were cultured without hydrogel exposure. J) Cellular viability after 1 and 5 days of incubation with either GelMAG or GDP hydrogels. K) Representative actin/DAPI stained images of NIH3T3 cells cultured with GelMAG or GDP for 5 days. L) Relative fluorescence unit (RFU) of cells cultured with GelMAG or GDP for 1, 3, and 5 days, assessed through PrestoBlue assay. Data are represented as mean ± SD. Analysis by two‐way ANOVA with Tukey's post‐hoc multiple comparisons test. * p < 0.05, ** p < 0.01, **** p < 0.0001. n = 3 biological replicates per group.

Article Snippet: In vitro biocompatibility studies were conducted using NIH3T3 fibroblast cells (catalog number CRL‐1658, 3T3‐ Swiss albino cell line, ATCC).

Techniques: In Vitro, Concentration Assay, Inhibition, Negative Control, Bacteria, Staining, Control, Cell Culture, Incubation, Fluorescence, Prestoblue Assay

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Regulation of HMGA1 expression by microRNA-296 affects prostate cancer growth and invasion

doi: 10.1158/1078-0432.CCR-10-0993

Figure Lengend Snippet: A. Photomicrographs illustrated examples of cell density in control (block-iT, top) and miR-296 mimic (bottom). B. Histobars illustrated the average cell counts through the Biocoat Matrigel invasion chamber in vitro (y-axis) in different treatments (x-axis, listed below). Small t-bars were standard errors.

Article Snippet: B. Histobars illustrated the average cell counts through the Biocoat Matrigel invasion chamber in vitro (y-axis) in different treatments (x-axis, listed below).

Techniques: Control, Blocking Assay, In Vitro

Journal: Oncotarget

Article Title: Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling

doi:

Figure Lengend Snippet: A–D. The indicated cell lines, in which DR5 was stably knocked down (A), knocked out (B), knocked down and then re-expressed (C) or knocked out and then re-expressed (D) as evaluated with Western blotting were plated in Matrigel invasion chambers for cell invasion assay and in 96-well plates for cell number estimation with MTS assay after approximately 48 h incubation. E. A549 cells transiently transfected with control (Ctrl), DR5 or DR4 siRNA were plated in 12-well plates for evaluation of DR5 or DR4 expression by Western blotting, in the Matrigel invasion chambers for invasion assay and in 96-well plates for cell number estimation after approximately 48 h incubation. The data are means ± SDs of triplicate (invasion) or quadruplicate (cell growth) determinations. WT, wild-type.

Article Snippet: The in vitro cell invasion assay was carried out in BD BioCoat Matrigel invasion chambers (Becton Dickinson).

Techniques: Stable Transfection, Western Blot, Invasion Assay, MTS Assay, Incubation, Transfection, Expressing

Journal: Oncotarget

Article Title: Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling

doi:

Figure Lengend Snippet: A and B. A549 cells transfected with control (Ctrl) or DR5 siRNA were plated in Matrigel invasion chambers for approximately 12 h and then exposed to the given concentrations of U0126 (A) or SP600125 (B) in the bottom wells for an additional 36 h. The invasive cells were stained and measured. In addition, cell growth in 96-well plates was measured with the MTS assay after exposure to U0126 or SP600125 for about 48 h. C–F. A549 cells were co-transfected with the indicated siRNAs alone or in combinations and then seeded in 12-well plates for Western blotting to confirm knockdown efficiencies, in Matrigel invasion chambers for cell invasion assays, and in 96-well plates for cell growth measurements after approximately 48 h incubation. The data are means ± SDs of triplicate (invasion) or quadruplicate (cell growth) determinations.

Article Snippet: The in vitro cell invasion assay was carried out in BD BioCoat Matrigel invasion chambers (Becton Dickinson).

Techniques: Transfection, Staining, MTS Assay, Western Blot, Incubation

Journal: Oncotarget

Article Title: Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling

doi:

Figure Lengend Snippet: A. and B. A549 cells were transfected with the indicated siRNAs alone or in combinations and after 48 h, were harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis. C. A549 cells transfected with the indicated siRNAs were seeded in 12-well plates for Western blotting, in Matrigel invasion chambers for cell invasion assay and in 96-well plates for cell growth measurements after approximately 48 h incubation. The data are means ± SDs of duplicate (invasion) or quadruplicate (cell growth) determinations.

Article Snippet: The in vitro cell invasion assay was carried out in BD BioCoat Matrigel invasion chambers (Becton Dickinson).

Techniques: Transfection, Western Blot, Invasion Assay, Incubation

Journal: Oncotarget

Article Title: Suppression of death receptor 5 enhances cancer cell invasion and metastasis through activation of caspase-8/TRAF2-mediated signaling

doi:

Figure Lengend Snippet: A and E. A549 cells transfected with the indicated siRNAs alone or in combinations were seeded in 12-well plates for Western blotting to detect the given proteins, in Matrigel invasion chambers for cell invasion assays and in 96-well plates for cell growth measurements after approximately 48 h incubation. The data are means ± SDs of triplicate (A) or duplicate (E) determinations (invasion) or quadruplicate determinations (cell growth). B and C , A549 (B) and HCT116 DR5-KO (C) cells transfected with control (Ctrl) or indicated siRNA were plated in Matrigel invasion chambers for approximately 12 h and then exposed to the given caspase inhibitors (20 μM) in the bottom wells for an additional 36 h. The invasive cells were stained and measured. In addition, the cells were also plated in 96-well plates and received the same treatments with the indicated caspase inhibitors for 48 h for measurement of cell growth with the MTS assay. The data are means ± SDs of triplicate (invasion) or quadruplicate (cell growth) determinations. D , HCT116 cells were seeded in 96-well plates and treated with 50 ng/ml TRAIL alone or in combination with 20 μM Z-VAD or Z-IETD. After 4 h, the cell viability was measured with the MTS assay. The data are means ± SDs of quadruplicate determinations.

Article Snippet: The in vitro cell invasion assay was carried out in BD BioCoat Matrigel invasion chambers (Becton Dickinson).

Techniques: Transfection, Western Blot, Incubation, Staining, MTS Assay